Good old budding yeast (Wiki Commons)

The Yeast Resource Center Public Image Repository (YRC PIR) is a large database of fluorescence microscopy images of yeast, with a focus on the important model organism Saccharomyces cerevisiae with some attention given to S. pombe.

• 84,270 Experiments
• 530,982 images
• ~450 gigabytes of data

Not bad.

A press release posted at 7th Space offers a short introduction:

There is increasing interest in the development of computational methods to analyze fluorescent microscopy images and enable automated large-scale analysis of the subcellular localization of proteins. Determining the subcellular localization is an integral part of identifying a protein’s function, and the application of bioinformatics to this problem provides a valuable tool for the annotation of proteomes.

NUF2 (Venus tag) and SPC97 (CFP tag) proteins in yeast

The YRC PIR is the work of Trisha Davis and Michael Riffle of the University of Washington and is supported by grant P41 RR11823 from the National Center for Research Resources and grant GM40506 from National Institute of General Medical Sciences at the US National Institutes of Health. Amazingly all the 500,000 + images in the database are from the Davis lab at U of W.

The database is of course online but can also be downloaded for local use. It can be searched using a variety of parameters including fluorescent tag.

For pretty much everything you need to know about this outstanding resource see the YRC PIR FAQ and user guide.